Recombinant Human VEGF

50µg / 1mg

OL13-50, OL13-1000 (187€ - 1,727€)

Vascular Endothelial Growth Factor (VEGF) is a ~19.2 kDa protein, consisting of 165 amino acids and with a potent angiogenic activity both in vitro and in vivo (in embryo and adult tissues). VEGF is a heparin-binding homodimeric glycoprotein that binds to VEGFR-1 (R1) and VEGFR-2 (R2), and activates Raf/MEK/ERK and PI3K/AKT pathways. It plays an important role in vasculogenesis and angiogenesis. It has also important effects during neurogenesis as a neurotrophic factor on dopaminergic neurons and astrocytes.

Bioactivity analysis

The biological activity of CB recombinant human VEGF165 was tested by its ability to promote cell proliferation of HUVEC cells, measured using non-radioactive colorimetric assay and monitored by absorbance. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. For this particular case, Core Bio rhVEGF provides better bioactivity when compared to a competitor standard supplier (derived from e.coli)

Purity analysis

~ 2 μ g / l a n e o f Recombinant Human VEGF was resolved in S D S - PAGE reduced ( R ) conditions. The image shows the purity of recombinant human VEGF with a predicted molecular mass of 19.2 kDa.

Typical Applications

CB recombinant human VEGF product is a highly potent growth factor ideal for stem cell research and bioengineering applications, including the proliferation and survival of endothelial cells for vascularized in vitro models, transformation of iPSCs into NK cells, growth of cells from the neural lineage, bone regeneration, and wound healing.

  • Alternative Names:

    MVCD1, VAS, Vasculotropin, VEGF-A, VEGFA, Vascular Permeability Factor (VPF).

    Accession Number:

    P15692

    Molecular mass:

    Estimated 19.2kDA.

    Origin:

    Plant-derived.

    Species:

    Human.

    Similarity:

    Porcine (97%), Bovine (95%), mouse (88%)

  • Purity:

    95% SDS page resolved under reduced (R) conditions.

    Bioactivity:

    EC5O ≤ 1 ng/ml.

    Formulation:

    Lyophilized.

    Endotoxin level:

    Recombinant protein expressed in plant system, free of bacterial endotoxins.

    Animal Component:

    Animal-derived Component Free. CB strictly guarantees that our recombinant proteins are not produced with or contain any components of animal origin. ADCF statement available

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