Recombinant Human KGF (FGF-7)

50µg / 1mg

OL14-50, OL14-1000 (287€ - 2,577€)

Keratinocyte Growth Factor (KGF) also known as Fibroblast Growth Factor 7 (FGF-7), is a potent mitogen factor of ~19.0kDa specific for epithelial cells and keratinocytes, and consisting of 163 amino acid residues.

As one of the key members of the FGF family, it signals through the FGF R2 (FGF R2- IIIb/KGF R) receptor. It is expressed by mesenchymal cells, and when secreted, it plays a crucial role to accelerate wound healing processes due to its up regulation in response to skin damage.

Bioactivity analysis

The biological activity of Core Bio Human Recombinant KGF was tested by its ability to promote cell proliferation of MCF-7 cells, measured using non-radioactive colorimetric assay and monitored by absorbance. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. For this case, Core Bio rhKGF corresponds to EC50=0.39 ng/mL, while for competitor standard e.coli derived rhKGF corresponds to EC50=1.89 ng/mL.

Purity analysis

~ 2 μ g / l a n e o f Recombinant Human KGF was resolved in S D S - PAGE reduced ( R ) conditions. The image shows the purity of recombinant human KGF with a predicted molecular mass of 19.0 kDa.

Typical Applications

Core Bio recombinant human KGF product is a highly pure and bioactive growth factor providing high performance and reproducible results among various stem cell culture and tissue engineering applications, including growth of epithelial cells and keratinocytes, formation of in vitro skin constructs, differentiation into insulin-producing cells, and research with patient derived cancer organoids of various tissues such us the lung and bladder.

  • Alternative Names:

    FGF-7, HBGF-7, Heparin-binding growth factor 7.

    Accession Number:

    P21781

    Molecular mass:

    Estimated 19.0kDA.

    Origin:

    Plant-derived.

    Species:

    Human.

    Similarity:

    Bovine (98%), Porcine (96%), mouse (95%)

  • Purity:

    95% SDS page resolved under reduced (R) conditions.

    Bioactivity:

    EC5O ≤ 1 ng/ml.

    Formulation:

    Lyophilized.

    Endotoxin level:

    Recombinant protein expressed in plant system, free of bacterial endotoxins.

    Animal Component:

    Animal-derived Component Free. CB strictly guarantees that our recombinant proteins are not produced with or contain any components of animal origin. ADCF statement available

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