Recombinant FGF-2 TOP® GMP
( Thermostability Optimized )
50µg / 1mg
FGF-2-TOP® (Thermostability Optimized) -formerly STAB- is a stabilized growth factor that offers a novel way to grow FGF-2-dependent cell cultures more efficiently, with fewer media changes. FGF2-TOP® retains full biological activity even after seven days at 37°C.
The stable levels of FGF-2 in culture allows for a more homogenous, undifferentiated stem cell culture, while saving cell therapy developers valuable time and money because repeated supplementation with FGF-2 and a daily medium change is not required. CB FGF-2 TOP® is the 155 aa mature domain of FGF-2 with nine amino acid substitutions to enhance stability without impacting bioactivity developed by Dvorak et al. 2018. This increases the functional half-life of the protein from <10 h (wild-type) to >7 days (FGF2-TOP®) in cell culture conditions at 37ºC.
Bioactivity analysis
Comparison of FGF2-TOP® from Core Biogenesis (plant-expressed) with human FGF-2 STAB (G3) reference standard (e.coli expressed) revealed that after 5 days of culturing 3T3 at 37ºC, cell proliferation FGF2-TOP® from Core Biogenesis is higher than the reference standard. FGF2-TOP® from Core Bio exhibited an EC50 value of 0.04ng/mL as compared to the e.coli derived product, corresponding to EC50 0.16ng/mL.
Purity analysis
1 μg/lane of CB recombinant human FGF-2 TOP® (Thermostable) was resolved in S D S - PA G E reduced ( R ) conditions. Showing a predicted molecular mass of 17.4 kDa.
Typical Applications
CB recombinant FGF-2 TOP® is the new gold-standard for high quality, reproducible and cost-effective stem cell cultures. It delivers superior performance with pluripotent stem cell cultures in both adherent and suspension conditions, while permitting to have better control over the desired cell phenotypes in downstream differentiations.
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Alternative Names:
FGF-2 G3, bFGF STAB, Thermostable FGF-2.
Accession Number:
155 amino acids with 9 aa point mutations from the wild-type FGF-2. Tag-free. Original sequence accession number P09038
Molecular mass:
Estimated 17.4 kDA.
Origin:
Plant-derived.
Species:
Engineered sequence.
Similarity:
Species neutral. Reactivity with Human, Bovine, Porcine and Mouse.
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Purity:
95% SDS page resolved under reduced (R) conditions.
Bioactivity:
EC5O ≤ 0.5 ng/ml.
Formulation:
Lyophilized.
Endotoxin level:
Recombinant protein expressed in plant system, free of bacterial endotoxins.
Animal Component:
Animal-derived Component Free. CB strictly guarantees that our recombinant proteins are not produced with or contain any components of animal origin. ADCF statement available
Key features
A novel nine amino acid substitution of the wild-type FGF-2 was performed, improving the heat stability of FGF-2 TOP®. This results in an increased half-life under cell culture conditions at 37°C, from < 10hours (wild-type FGF-2) to > 7 days (FGF-2 TOP®). The consistent signalling and enhanced protein bioactivity is directly translated into better phenotype control, and homogeneity in stem cell cultures.
Seamless transition
From RUO to GMP-Compatible materials
Specifications
Origin
FGF-2 TOP® is produced by recombinant expression in plant seeds
Identity
HPLC
Bioactivity
EC50, IUs
Purity
>97%
Concentration
1mg/mL (Liquid format)
Mycoplasma
Negative by PCR
Bioburden
USP <61>
Endotoxin
USP <85> (< 50EU/mL)
Shelf-life
Stability studies >24 months
Release
CoA
Storage
-80º C
Our process offers scalable, cost-effective, and carbon-negative materials production. Seed yield and storage provide a sustainable solution for supply chain consistency. FGF-2 TOP® is manufactured under cGMP conditions and governed by a robust quality management system in collaboration with our partner, Nucleus Biologics.
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