Recombinant FGF-2 TOP®

( Thermostability Optimized )

50µg / 1mg

PL9-50, PL9-1000 (247€ - 729€)

FGF-2-TOP® (Thermostability Optimized) -formerly STAB- is a stabilized growth factor that offers a novel way to grow FGF-2-dependent cell cultures more efficiently, with fewer media changes. FGF2-TOP® retains full biological activity even after seven days at 37°C. The stable levels of FGF-2 in culture allows for a more homogenous, undifferentiated stem cell culture, while saving researchers valuable time and money because repeated supplementation with FGF-2 and a daily medium change is not required. CB FGF-2 TOP® is the 155 aa mature domain of FGF-2 with nine amino acid substitutions to enhance stability without impacting bioactivity developed by Dvorak et al. 2018. This increases the functional half-life of the protein from <10 h (wild-type) to >7 days (FGF2-TOP®) in cell culture conditions at 37ºC.

Bioactivity analysis

Comparison of FGF2-TOP® from Core Biogenesis (plant-expressed) with human FGF-2 STAB (G3) reference standard (e.coli expressed) revealed that after 5 days of culturing 3T3 at 37ºC, cell proliferation FGF2-TOP® from Core Biogenesis is higher than the reference standard. FGF2-TOP® from Core Bio exhibited an EC50 value of 0.04ng/mL as compared to the e.coli derived product, corresponding to EC50 0.16ng/mL.

Purity analysis

1 μg/lane of CB recombinant human FGF-2 TOP® (Thermostable) was resolved in S D S - PA G E reduced ( R ) conditions. Showing a predicted molecular mass of 17.4 kDa.

Typical Applications

CB recombinant FGF-2 TOP® is the new gold-standard for high quality, reproducible and cost-effective stem cell cultures. It delivers superior performance with pluripotent stem cell cultures in both adherent and suspension conditions, while permitting to have better control over the desired cell phenotypes in downstream differentiations.

  • Alternative Names:

    FGF-2 G3, bFGF STAB, Thermostable FGF-2.

    Accession Number:

    155 amino acids with 9 aa point mutations from the wild-type FGF-2. Tag-free. Original sequence accession number P09038

    Molecular mass:

    Estimated 17.4 kDA.

    Origin:

    Plant-derived.

    Species:

    Engineered sequence.

    Similarity:

    Species neutral. Reactivity with Human, Bovine, Porcine and Mouse.

  • Purity:

    95% SDS page resolved under reduced (R) conditions.

    Bioactivity:

    EC5O ≤ 0.5 ng/ml.

    Formulation:

    Lyophilized.

    Endotoxin level:

    Recombinant protein expressed in plant system, free of bacterial endotoxins.

    Animal Component:

    Animal-derived Component Free. CB strictly guarantees that our recombinant proteins are not produced with or contain any components of animal origin. ADCF statement available

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