Recombinant Human FGF-2

50µg / 1mg

PL1-50, PL1-1000 (197€ - 597€)

FGF-2, also known as basicFGF, is a growth factor that is produced mainly in fibroblasts, endothelial cells and smooth muscle cells. FGF-2 controls fundamental biological processes, including cell growth and differentiation, tissue formation, angiogenesis and wound healing. It is also an essential component in human pluripotent stem cells culture, as one of the main media components for maintaining pluripotency. Recombinant human FGF-2 consists of 146 amino acids, 16.06 kDa. FGF-2 is qualified for different cell stem cell culture applications, and cell-manufacturing technologies.

Bioactivity analysis

The biological activity of CB recombinant human FGF-2 was tested by its ability to promote the proliferation of NIH/3T3 fibroblasts. Cell proliferation is measured using non-radioactive colorimetric assay and monitored by absorbance. The EC50 is generally ≤0.5 ng/mL. For this particular case, CB hrFGF-2 corresponds to EC50=0.36 ng/mL.

Purity analysis

1 μg/lane of CB recombinant human FGF-2 was resolved in S D S - PA G E reduced ( R ) conditions. Showing a predicted molecular mass of 16.4 kDa.

Typical Applications

CB recombinant human FGF-2 is a potent growth factor with high bioactivity in cultures employing pluripotent (PSCs/iPSCs), multipotent (Mesenchymal Stem Cells), and tissue-specific stem cells from various tissues such as neural, dermal and pancreatic.

  • Alternative Names:

    bFGF, FGF-β, FGF2, Fibroblast growth factor-basic, HBGF-2.

    Accession Number:

    P09038

    Molecular mass:

    Estimated 16.4kDA.

    Species:

    Human.

    Similarity:

    Bovine (99%), Porcine (99%), Mouse (94%).

  • Purity:

    95% SDS page resolved under reduced (R) conditions.

    Bioactivity:

    EC5O ≤ 1 ng/ml.

    Formulation:

    Lyophilized.

    Endotoxin level:

    Recombinant protein expressed in plant system, free of bacterial endotoxins.

    Animal Component:

    Animal-derived Component Free. CB strictly guarantees that our recombinant proteins are not produced with or contain any components of animal origin. ADCF statement available

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