Recombinant Human EGF

50µg / 1mg

OL3-50, OL3-1000 (57€ - 167€)

Epidermal Growth Factor (EGF) is a potent mitogenic growth factor ~6 kDa protein, consisting of a single polypeptide of 53 amino acid residues and three intramolecular disulfide bonds that stimulates cell growth, proliferation and differentiation, oncogenesis and wound healing. EGF exerts its effects in the target cells by binding to the transmembrane protein tyrosine kinase EGF receptor. EGF proteins are evolutionarily conserved across many species.

Bioactivity analysis

The biological activity of CB Recombinant Human EGF was tested by its ability to promote the proliferation of BALB/c 3T3 cells. Cell proliferation was measured using non-radioactive colorimetric assay and monitored by absorbance. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. For this particular case, Core Bio rhEGF corresponds to EC50=0.34 ng/mL, while for competitor e.coli derived rEGF corresponds to EC50=0.99 ng/mL.

Purity analysis

~ 2 μ g / l a n e o f Recombinant Human EGF was resolved in S D S - PAGE reduced ( R ) conditions. The image shows the purity of recombinant human EGF with a predicted molecular mass of 6.2 kDa.

Typical Applications

CB recombinant human EGF product is a highly pure and bioactive growth factor providing high performance and reproducible results among various stem cell culture, and cell manufacturing applications, including direct differentiation of hepatic progenitors into mature hepatocytes, expansion of neural progenitors, formation of germ cells, formation of intestinal organoids, as well as in wound healing studies, tissue engineering, and cancer research.

  • Alternative Names:

    Epidermal growth factor, Pro-epidermal growth factor, URG, Urogastrone.

    Accession Number:

    Q6QBS2

    Molecular mass:

    Estimated 6.2kDA.

    Origin:

    Plant-derived.

    Species:

    Human.

    Similarity:

    Porcine (85%).

  • Purity:

    95% SDS page resolved under reduced (R) conditions.

    Bioactivity:

    EC5O ≤ 1 ng/ml.

    Formulation:

    Lyophilized.

    Endotoxin level:

    Recombinant protein expressed in plant system, free of bacterial endotoxins.

    Animal Component:

    Animal-derived Component Free. CB strictly guarantees that our recombinant proteins are not produced with or contain any components of animal origin. ADCF statement available

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